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微信扫一扫Use the primers 27F (5'-AGA-GTT-TGA-TCC-TGG-CTC-AG-3') and 1,492R (5'-GGT-TAC-CTT-GTT-ACG-ACT-T-3') to perform PCR amplification with annealing temperature at 52℃. The amplicon size is 1,500bp. Perform sequencing on the amplicon for confirmation.
Use the primers 27F (5'-AGA-GTT-TGA-TCC-TGG-CTC-AG-3') and 1,492R (5'-GGT-TAC-CTT-GTT-ACG-ACT-T-3') to perform PCR amplification with annealing temperature at 52℃. The amplicon size is 1,500bp. Perform sequencing on the amplicon for confirmation.
