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2.3 Fluorescent and CBB gel staining and image analysis

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摘要

After 2-DE, the gels were fixed,followed by staining with Pro-Q Diamond(Invitrogen, Eugene, OR)to detect phosphoproteins. The total proteins were visualized by CBB G-250.The best three gels of each sample were selected for subsequent gel analysis using Progenesis SameSpots(v 4.1,Nonlinear Dynamic, U