注册

2.3 Preparation of protein samples

字体大小: 点赞 

摘要

Protein extraction was according to Li et al.(2007).Hypopharyngeal glands(1mg HG/10μL buffer)were added to phosphate buffer(PB)pH 7.6,containing 32.5mMol/L K2HPO4,2.6mMol/L KH2PO4,400mMol/L NaCl. The glands were homogenized for 20minutes on ice and sonicated for 2minutes,then centrifuged at 12000g a