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2.3 Two-Dimensional Gel Electrophoresis(2-DE)and Image Statistical Analysis

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摘要

Hemolymph protein extraction was carried out according to a previously described method. A 558μg protein sample from each larval and pupal sample with three biological replicates(which were pooled together from five honeybee colonies)was suspended in 90μL of lysis buffer(8 M urea,2 M thiourea,4% CHA