QQ好友
新浪微博
微信扫一扫Protein extraction was carried out according to the method of Zhang et al.（2007）with some improvements. The eggs（10μL buffer per 1mg eggs）were mixed with PBS pH 7.6，containing 32.5mmol L-1 K2HPO4，2.6mmol L-1 KH2PO4，and 400mmol L-1 NaCl. The mixture was homogenized 20min in the ice and sonicated 2min
